亚洲成人涩涩 I 在线播放你懂的 I 污网站在线观看视频 I 日本国产中文字幕 I 久久久久久国产 I 六月婷综合 I av 一区 二区 久久 I 日本丰满熟妇hd I 一级黄片毛片 I 国产一级久久 I 欧美性精品220 I 日本肉感肥臀一二三区 I www国产在线 I 欧美日韩中文另类 I 国产一区二区不卡在线看 I 亚洲国产成人不卡 I 亚洲日本精品一区 I 篠田优中文在线播放第一区 I 国产二区视频在线播放 I 性猛aa久久久 I 成在人av抽搐高潮喷水流白浆 I 日韩aa大片 I 噜噜噜噜噜久久久久久91黄画 I 黄页网站在线看 I 欧美精品久久99久久在免费线 I 18岁禁看奶视频免费看 I 亚洲中文欧美在线视频 I 国产做爰xxxⅹ久久久 I 欧美一区二区视频在线观看 I wwwxxx黄色 I 国产精品12区

sigmaDL-二硫蘇糖醇DL-Dithiothreitol

CAS number: 3483-12-3
Product Description:
Appearance: White Powder
Molecular formula: C4H10O2S2
Melting Point: 42-43°C
Molecular weight: 154.1 g/mol
Solubility: 0.1 M in H2O, 20°C, complete, colorless
pH: 4.0-6.5 （0.1 M in H2O, 25°C）
This product is designated as BioChemika Ultra for Molecular Biology grade and is suitable for applications in the molecular biology, specially if high purity is required. It has been analyzed for low contaminating impurity and the absence of nucleases.
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 （1949）
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB （Fluka 43760）.2
Applications:
It is used in buffers for isolation of RNA5 or buffers used for RT-PCR starting with mRNA6,7.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether.
Solutions oxidize relatively slowly in air.3 It is recommended to make solutions fresh daily. Recorded half-life （hours） of DTT solutions at various pH and temperatures are as follows:4
Conditions （all in 0.1 M KPO4 buffer）
Half life （Hours）
pH 6.5 at 20°C
40
pH 7.5 at 20°C
10
pH 8.5 at 20°C
1.4
pH 8.5 at 0°C
11
pH 8.5 at 40°C
0.2
pH 8.5 at 20°C +0.1 mM Cu2+
0.6
pH 8.5 at 20°C +0.1 mM EDTA
4
Storage/Stability:
Storage Temperature: 4°C
When stored as indicated, this product is stable for years.

上海力敏實業有限公司代理sigma產品，咨詢sigmaDL-二硫蘇糖醇DL-Dithiothreitol 詳細產品信息